PAPSS1 Antibody (1F4)
Novus Biologicals, part of Bio-Techne | Catalog # H00009061-M05
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PAPSS1 (NP_005434, 144 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAE
Reactivity Notes
Human. Other species not tested.
Specificity
PAPSS1 - 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for PAPSS1 Antibody (1F4)
![Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05]](https://resources.bio-techne.com/images/products/PAPSS1-Antibody-1F4-Western-Blot-H00009061-M05-img0004.jpg "Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05]")
Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05]
Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody (M05), clone 1F4. Lane 1: PAPSS1 transfected lysatE (68.5 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody (1F4) [H00009061-M05]](https://resources.bio-techne.com/images/products/PAPSS1-Antibody-1F4-Immunocytochemistry-Immunofluorescence-H00009061-M05-img0003.jpg "Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody (1F4) [H00009061-M05]")
Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody (1F4) [H00009061-M05]
Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of monoclonal antibody to PAPSS1 on HeLa cell. Antibody concentration 10 ug/ml![Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05]](https://resources.bio-techne.com/images/products/PAPSS1-Antibody-1F4-Western-Blot-H00009061-M05-img0002.jpg "Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05]")
Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05]
Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi or non-transfected control. Blot probed with H00009061-M05. GAPDH (36.1 kDa) used as loading control.Applications for PAPSS1 Antibody (1F4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validaton and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PAPSS1
Alternate Names
3'-phosphoadenosine 5'-phosphosulfate synthase 1, ATPSK1SK 1, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, EC 2.7.1.25, PAPS synthase 1, PAPSS 1, PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1, SK1, Sulfurylase kinase 1
Entrez Gene IDs
9061 (Human)
Gene Symbol
PAPSS1
OMIM
603262 (Human)
UniProt
Additional PAPSS1 Products
Product Documents for PAPSS1 Antibody (1F4)
Product Specific Notices for PAPSS1 Antibody (1F4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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