PAR2 Antibody (9X8E6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16555
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9X8E6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 298-397 of human PAR2 (P55085). ICFTPSNLLLVVHYFLIKSQGQSHVYALYIVALCLSTLNSCIDPFVYYFVSHDFRDHAKNALLCRSVRTVKQMQVSLTSKKHSRKSSSYSSSSTTVKTSY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PAR2 Antibody (9X8E6)
Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555]
Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of various cell lines, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunohistochemistry-Paraffin: PAR2 Antibody (9X8E6) [NBP3-16555]
Immunohistochemistry-Paraffin: PAR2 Antibody (9X8E6) [NBP3-16555] - Human colon carcinoma using PAR2 Rabbit mAb (NBP3-16555) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555]
Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of Rat large intestine, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.Applications for PAR2 Antibody (9X8E6)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PAR2
Long Name
Protease-Activated Receptor 2
Alternate Names
CFIIRL1, F2RL1, GPCR11
Gene Symbol
F2RL1
Additional PAR2 Products
Product Documents for PAR2 Antibody (9X8E6)
Product Specific Notices for PAR2 Antibody (9X8E6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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