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PAR4 Antibody (5F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00009002-M01

Novus Biologicals, part of Bio-Techne
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H00009002-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

F2RL3 (NP_003941, 18 a.a. ~ 82 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGTQTPSVYDESGSTGGGDDSTPSILPAPRGYPGQVCANDSDTLELPDSSRALLLGWVPTRLVPA

Localization

Integral Memebrane Protein

Specificity

F2RL3 - coagulation factor II (thrombin) receptor-like 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PAR4 Antibody (5F4)

ELISA: PAR4 Antibody (5F4) [H00009002-M01]

ELISA: PAR4 Antibody (5F4) [H00009002-M01]

ELISA: PAR4 Antibody (5F4) [H00009002-M01] - Detection limit for recombinant GST tagged F2RL3 is approximately 0.03ng/ml as a capture antibody.

Applications for PAR4 Antibody (5F4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAR4

Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin.

Long Name

Protease-Activated Receptor 4

Alternate Names

F2RL3, Protease-Activated Receptor 4, Thrombin Receptor-like 3

Entrez Gene IDs

9002 (Human)

Gene Symbol

F2RL3

OMIM

602779 (Human)

Additional PAR4 Products

Product Documents for PAR4 Antibody (5F4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAR4 Antibody (5F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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