Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PARC Antibodies »

Product Specifications

Immunogen

A synthetic peptide, mapping to a region between residue 2475 and the C-terminus (residue 2527) of human P53-associated parkin-like cytoplasmic protein using the numbering given in TrEMBL entry Q8IWT3 (GeneID 23113).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARC Antibody

Western Blot: PARC Antibody [NB200-187]

Western Blot: PARC Antibody [NB200-187]

Western Blot: PARC Antibody [NB200-187] - Detection of Human PARC on HeLa whole cell lysate using NB200-187. PARC was also immunoprecipited using NB200-186. Immunoprecipitated PARC was blotted using NB200-187.

Applications for PARC Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:20000-1:30000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PARC

PARC is a cytoplasmic anchor protein in p53 associated protein complexes. PARC directly interacted and formed an approximately 1MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53 dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53. Furthermore, abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.

Alternate Names

CUL-9, cullin 9, cullin-9, DKFZp686G1042, H7AP1DKFZp686P2024, p53-associated parkin-like cytoplasmic protein, PARCKIAA0708, parkin-like cytoplasmic p53 binding protein, RP3-330M21.2, UbcH7-associated protein 1

Entrez Gene IDs

23113 (Human); 78309 (Mouse)

Gene Symbol

CUL9

UniProt

Q8IWT3 (Human)

Additional PARC Products

Product Documents for PARC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PARC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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