PARD6A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38487

Novus Biologicals, part of Bio-Techne
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NBP2-38487
NBP2-38487-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PARD6A Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DLVIENRQPPSSNGLSQGPPCWDLHPGCRHPGTRSSLPSLDDQEQASSGWGSRIRGDGSGFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARD6A Antibody

Western Blot: PARD6A Antibody [NBP2-38487]

Western Blot: PARD6A Antibody [NBP2-38487]

Western Blot: PARD6A Antibody [NBP2-38487] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: PARD6A Antibody [NBP2-38487]

Immunocytochemistry/ Immunofluorescence: PARD6A Antibody [NBP2-38487]

Immunocytochemistry/Immunofluorescence: PARD6A Antibody [NBP2-38487] - Staining of human cell line MCF7 shows localization to actin filaments & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PARD6A Antibody [NBP2-38487]

Immunohistochemistry-Paraffin: PARD6A Antibody [NBP2-38487]

Immunohistochemistry-Paraffin: PARD6A Antibody [NBP2-38487] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
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Applications for PARD6A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARD6A

his gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

PAR-6, PAR-6 alpha, par-6 partitioning defective 6 homolog alpha (C. elegans), PAR6A, PAR6alpha, PAR-6Apar-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6C, partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6, Tax interaction protein 40, TAX40, Tax-interacting protein 40, TIP-40PAR6

Gene Symbol

PARD6A

UniProt

Q9NPB6 (Human)

Additional PARD6A Products

Product Documents for PARD6A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PARD6A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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