PARG1 Antibody

Western Blot: PARG1 Antibody [NBP3-12573]

Western Blot: PARG1 Antibody [NBP3-12573] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:142 kDaObserved band size:170 kDa

Immunocytochemistry/ Immunofluorescence: PARG1 Antibody [NBP3-12573]

Immunocytochemistry/Immunofluorescence: PARG1 Antibody [NBP3-12573] - Staining of ARHGAP29 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ARHGAP29 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm

Immunoprecipitation: PARG1 Antibody [NBP3-12573]

Immunoprecipitation: PARG1 Antibody [NBP3-12573] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-ARHGAP29 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ARHGAP29 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 142 kDaObserved band size :171 kDa