Park7/DJ-1 Antibody (007)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05805
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 007
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Park7/DJ-1 (Uniprot#: Q99497-1; Met1-Asp189).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Park7/DJ-1 Antibody (007)
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805]
Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Staining of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805]
Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Analysis of Human PARK7 expression on HeLa cells. The cells were treated, stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805]
Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PARK7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARK7 rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDaApplications for Park7/DJ-1 Antibody (007)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Park7/DJ-1
PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
Additional Park7/DJ-1 Products
Product Documents for Park7/DJ-1 Antibody (007)
Product Specific Notices for Park7/DJ-1 Antibody (007)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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