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Park7/DJ-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54654

Novus Biologicals, part of Bio-Techne
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NBP2-54654
NBP2-54654-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: GKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Park7/DJ-1 Antibody

Western Blot: Park7/DJ-1 Antibody [NBP2-54654]

Western Blot: Park7/DJ-1 Antibody [NBP2-54654]

Western Blot: Park7/DJ-1 Antibody [NBP2-54654] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NBP2-54654]

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NBP2-54654]

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NBP2-54654] - Staining of human pancreas shows cytoplasmic and nuclear positivity in exocrine glandular cells.
Western Blot: Park7/DJ-1 Antibody [NBP2-54654]

Western Blot: Park7/DJ-1 Antibody [NBP2-54654]

Western Blot: Park7/DJ-1 Antibody [NBP2-54654] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted) Lane 5: Human liver tissue Lane 6: Human tonsil tissue

Applications for Park7/DJ-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Park7/DJ-1

PARK7, also known as DJ-1, is a member of the peptidase C56 protein family. The PARK-7 protein is a positive regulator of androgen receptor-dependent transcription. It has also been reported to be a potential sensor for oxidative stress, a redox-sensitive chaperone, and a key protein for preventing neuronal oxidative stress and cell death.

PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.

Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Gene Symbol

PARK7

Additional Park7/DJ-1 Products

Product Documents for Park7/DJ-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Park7/DJ-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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