Parkin Antibody (JF82-09)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67017

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF82-09

Concentration

1 mg/ml

View all Parkin Antibodies »

Product Specifications

Immunogen

Synthetic peptide within N-terminal human Parkin. (SwissProt: O60260 Human; SwissProt: Q9WVS6 Mouse; SwissProt: Q9JK66 Rat)

Localization

Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Parkin Antibody (JF82-09)

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017]

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017]

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017]

Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017]

Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.
Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017]

Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017]

Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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Applications for Parkin Antibody (JF82-09)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

IF-Tissue-1:50-1:200, IF-Cell-1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

Use at an assay dependent concentration

Western Blot

1:500-1:1000

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-67017 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Parkin

Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Gene Symbol

PRKN

Additional Parkin Products

Product Documents for Parkin Antibody (JF82-09)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Parkin Antibody (JF82-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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