Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PARL Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL. The immunogen is located within amino acids 40 - 90 of PARL. Amino Acid Squence: RKAPRKVEPRRSDPG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARL Antibody - BSA Free

Western Blot: PARL AntibodyBSA Free [NBP1-76938]

Western Blot: PARL AntibodyBSA Free [NBP1-76938]

Western Blot: PARL Antibody [NBP1-76938] - 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 ug/ml.
PARL Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PARL Antibody - BSA Free [NBP1-76938] -

Immunocytochemistry/ Immunofluorescence: PARL Antibody - BSA Free [NBP1-76938] - Immunofluorescence of PARL in 3T3 cells with PARL antibody at 2.50 ug/mL.
PARL Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PARL Antibody - BSA Free [NBP1-76938] -

Immunocytochemistry/ Immunofluorescence: PARL Antibody - BSA Free [NBP1-76938] - Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 ug/mL.

Applications for PARL Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5-20 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARL

PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL's association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer's disease through its associations with presenilin-1 and -2. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Multiple isoforms of PARL are known to exist.

Long Name

Presenilins Associated Rhomboid-like Protein

Alternate Names

PSARL1, RHBDS1

Entrez Gene IDs

55486 (Human)

Gene Symbol

PARL

UniProt

Q9H300 (Human)

Additional PARL Products

Product Documents for PARL Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PARL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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