PARP Antibody (2G8N7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15802

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15802-100ul
NBP3-15802-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2G8N7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PARP Antibodies »

Product Summary for PARP Antibody (2G8N7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PARP (P09874). KLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARP Antibody (2G8N7)

Western Blot: PARP Antibody (2G8N7) [NBP3-15802]

Western Blot: PARP Antibody (2G8N7) [NBP3-15802]

Western Blot: PARP Antibody (2O6H4) [NBP3-15802] - Western blot analysis of extracts of Jurkat cells, using PARP antibody (NBP3-15802) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: PARP Antibody (2G8N7) [NBP3-15802]

Immunohistochemistry-Paraffin: PARP Antibody (2G8N7) [NBP3-15802]

Immunohistochemistry-Paraffin: PARP Antibody (2O6H4) [NBP3-15802] - Immunohistochemistry of paraffin-embedded mouse spleen using PARP antibody (NBP3-15802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: PARP Antibody (2G8N7) [NBP3-15802]

Western Blot: PARP Antibody (2G8N7) [NBP3-15802]

Western Blot: PARP Antibody (2O6H4) [NBP3-15802] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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Applications for PARP Antibody (2G8N7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARP

Poly (ADP-ribose) polymerase (PARP) is protein family primarily invloved in DNA repair and programmed cell death. PARP proteins are activated in response to DNA single strand breaks (SSBs). Upon SSB detection, PARP binds the DNA and synthesizes a PAR chain at the damaged site, which then signals the initiation of DNA repair by other enzymes such as XRCC1, DNA ligase III and DNA polymerase beta. After repair, the PAR chains are degraded via PAR glycohydrolase.

PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Gene Symbol

PARP1

Additional PARP Products

Product Documents for PARP Antibody (2G8N7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PARP Antibody (2G8N7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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