PARP Antibody (4M9X4) - Cleaved

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15813

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15813-100ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4M9X4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PARP Antibodies »

Product Summary for PARP Antibody (4M9X4) - Cleaved

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-214 of human PARP (P09874). QLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARP Antibody (4M9X4) - Cleaved

Western Blot: PARP Antibody (4M9X4)Cleaved [NBP3-15813]

Western Blot: PARP Antibody (4M9X4)Cleaved [NBP3-15813]

Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for PARP Antibody (4M9X4) - Cleaved

Application
Recommended Usage

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARP

Poly (ADP-ribose) polymerase (PARP) is protein family primarily invloved in DNA repair and programmed cell death. PARP proteins are activated in response to DNA single strand breaks (SSBs). Upon SSB detection, PARP binds the DNA and synthesizes a PAR chain at the damaged site, which then signals the initiation of DNA repair by other enzymes such as XRCC1, DNA ligase III and DNA polymerase beta. After repair, the PAR chains are degraded via PAR glycohydrolase.

PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Gene Symbol

PARP1

Additional PARP Products

Product Documents for PARP Antibody (4M9X4) - Cleaved

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PARP Antibody (4M9X4) - Cleaved

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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