PARP Antibody (8C7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26439
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8C7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PARP Antibody (8C7)
Immunogen
A synthesized peptide derived from Human PARP [UniProt P09874]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PARP Antibody (8C7)
Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] -
Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] -
Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: PARP Antibody (8C7) [NBP3-26439] -
Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate.All lanes: PARP Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 114 KDa
Observed band size: 114 kDa
Applications for PARP Antibody (8C7)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PARP
PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Gene Symbol
PARP1
Additional PARP Products
Product Documents for PARP Antibody (8C7)
Product Specific Notices for PARP Antibody (8C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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