PARP Antibody (CL2220)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36747

Novus Biologicals, part of Bio-Techne
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NBP2-36747
NBP2-36747-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2220

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PARP Antibodies »

Product Summary for PARP Antibody (CL2220)

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:KGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAM

Epitope

SKLPKPVQDL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PARP Antibody (CL2220)

Western Blot: PARP Antibody (CL2220) [NBP2-36747]

Western Blot: PARP Antibody (CL2220) [NBP2-36747]

Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747]

Immunocytochemistry/ Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747]

Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U251 cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747]

Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747]

Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
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Applications for PARP Antibody (CL2220)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARP

Poly (ADP-ribose) polymerase (PARP) is protein family primarily invloved in DNA repair and programmed cell death. PARP proteins are activated in response to DNA single strand breaks (SSBs). Upon SSB detection, PARP binds the DNA and synthesizes a PAR chain at the damaged site, which then signals the initiation of DNA repair by other enzymes such as XRCC1, DNA ligase III and DNA polymerase beta. After repair, the PAR chains are degraded via PAR glycohydrolase.

PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Gene Symbol

PARP1

UniProt

P09874

Additional PARP Products

Product Documents for PARP Antibody (CL2220)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PARP Antibody (CL2220)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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