PARP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06563

Novus Biologicals, part of Bio-Techne
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NBP1-06563
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PARP4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1674 to 1724 of human vault poly(ADP-ribose) polymerase using the numbering given in entry NP_006428.2 (GeneID 143).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARP4 Antibody

Western Blot: PARP4 Antibody [NBP1-06563]

Western Blot: PARP4 Antibody [NBP1-06563]

Western Blot: PARP4 Antibody [NBP1-06563] - Whole cell lysate from HeLa cells. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody NBP1-06562, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: PARP4 Antibody [NBP1-06563]

Immunohistochemistry-Paraffin: PARP4 Antibody [NBP1-06563]

Immunohistochemistry-Paraffin: PARP4 Antibody [NBP1-06563] - Human colon carcinoma. Antibody: Affinity purified rabbit anti- vPARP used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for PARP4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PARP4

This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq]

Long Name

Protein mono-ADP-ribosyltransferase PARP4

Alternate Names

ADPRTL1, ARTD4, KIAA0177, PARP-4, PH5P, VPARP

Entrez Gene IDs

143 (Human)

Gene Symbol

PARP4

UniProt

NP_006428.2 (Human)

Additional PARP4 Products

Product Documents for PARP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PARP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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