PASK Antibody (OTI8G9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73249

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI8G9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PASK Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PASK (NP_055963) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

142.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PASK Antibody (OTI8G9) - Azide and BSA Free

Western Blot: PASK Antibody (OTI8G9)Azide and BSA Free [NBP2-73249]

Western Blot: PASK Antibody (OTI8G9)Azide and BSA Free [NBP2-73249]

Western Blot: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PASK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK.
Immunocytochemistry/ Immunofluorescence: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249]

Immunocytochemistry/ Immunofluorescence: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249]

Immunocytochemistry/Immunofluorescence: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PASK.
Immunohistochemistry: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249]

Immunohistochemistry: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249]

Immunohistochemistry: PASK Antibody (OTI8G9) - Azide and BSA Free [NBP2-73249] - Staining of paraffin-embedded Human liver tissue using anti-PASK mouse monoclonal antibody.
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Applications for PASK Antibody (OTI8G9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PASK

PASKIN (PAS-Kinase) is thought to regulate protein synthesis in cells. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.

Alternate Names

DKFZp434O051, EC 2.7.11.1, hPASK, KIAA0135DKFZp686P2031, PAS domain containing serine/threonine kinase, PAS-kinase, PASKINPAS domain-containing serine/threonine-protein kinase, PAS-serine/threonine kinase, STK37

Gene Symbol

PASK

Additional PASK Products

Product Documents for PASK Antibody (OTI8G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PASK Antibody (OTI8G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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