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Patched 1/PTCH Antibody (PSH03-53)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32718

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32718

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH03-53

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within human Patched 1/PTCH aa 122-436 / 1,447. (Uniprot: Q13635)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Patched 1/PTCH Antibody (PSH03-53)

Patched 1/PTCH Antibody (PSH03-53)

Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718]

Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718] - Western blot analysis of Patched 1/PTCH on different lysates with Rabbit anti-Patched 1/PTCH antibody (NBP3-32718) at 1/10,000 dilution.

Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-PTCH1 cell lysate

Lysates/proteins at 1 ug/Lane.

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32718) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Patched 1/PTCH Antibody (PSH03-53)

Application
Recommended Usage

Western Blot

1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Patched 1/PTCH

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Gene Symbol

PTCH1

Additional Patched 1/PTCH Products

Product Documents for Patched 1/PTCH Antibody (PSH03-53)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Patched 1/PTCH Antibody (PSH03-53)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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