Patched 1/PTCH Antibody (PSH03-53)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32718
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-53
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human Patched 1/PTCH aa 122-436 / 1,447. (Uniprot: Q13635)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Patched 1/PTCH Antibody (PSH03-53)
Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718]
Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718] - Western blot analysis of Patched 1/PTCH on different lysates with Rabbit anti-Patched 1/PTCH antibody (NBP3-32718) at 1/10,000 dilution.Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-PTCH1 cell lysate
Lysates/proteins at 1 ug/Lane.
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32718) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Patched 1/PTCH Antibody (PSH03-53)
Application
Recommended Usage
Western Blot
1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Patched 1/PTCH
Alternate Names
BCNS, HPE7, mes, NBCCS, ptc1, PTCH1
Gene Symbol
PTCH1
Additional Patched 1/PTCH Products
Product Documents for Patched 1/PTCH Antibody (PSH03-53)
Product Specific Notices for Patched 1/PTCH Antibody (PSH03-53)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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