Patched 1/PTCH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41101

Novus Biologicals, part of Bio-Techne
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NB100-41101
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Patched 1/PTCH Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HPESRHHPPSNPRQQ corresponding to internal region according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.

Specificity

This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Patched 1/PTCH Antibody

Western Blot: Patched 1/PTCH Antibody [NB100-41101]

Western Blot: Patched 1/PTCH Antibody [NB100-41101]

Western Blot: Patched 1/PTCH Antibody [NB100-41101] - Staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-41101]

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-41101]

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-41101] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Patched 1/PTCH Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx 150 kDa band observed in human brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Patched 1/PTCH

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis. This gene functions as a tumor suppressor. Mutations of this gene have been associated with nevoid basal cell carcinoma syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Entrez Gene IDs

5727 (Human)

Gene Symbol

PTCH1

UniProt

NP_000255.1 (Human)

Additional Patched 1/PTCH Products

Product Documents for Patched 1/PTCH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Patched 1/PTCH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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