Pax3 Antibody (HL160)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13661
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Tagged Protein Expression
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL160
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to Coturnix coturnix Pax3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Pax3 Antibody (HL160)
![Western Blot: Pax3 Antibody (HL160) [NBP3-13661]](https://resources.bio-techne.com/images/products/Pax3-Antibody-HL160-Western-Blot-NBP3-13661-img0003.jpg "Western Blot: Pax3 Antibody (HL160) [NBP3-13661]")
Western Blot: Pax3 Antibody (HL160) [NBP3-13661]
Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661]](https://resources.bio-techne.com/images/products/Pax3-Antibody-HL160-Immunohistochemistry-Paraffin-NBP3-13661-img0002.jpg "Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661]")
Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661]
Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661] - Pax3 antibody [HL160] detects Pax3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. Pax3 stained by Pax3 antibody [HL160] (NBP3-13661) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: Pax3 Antibody (HL160) [NBP3-13661]](https://resources.bio-techne.com/images/products/Pax3-Antibody-HL160-Western-Blot-NBP3-13661-img0001.jpg "Western Blot: Pax3 Antibody (HL160) [NBP3-13661]")
Western Blot: Pax3 Antibody (HL160) [NBP3-13661]
Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Applications for Pax3 Antibody (HL160)
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pax3
Long Name
Paired Box Gene 3 [Waardenburg Syndrome 1]
Alternate Names
HUP2, WS1
Gene Symbol
PAX3
Additional Pax3 Products
Product Documents for Pax3 Antibody (HL160)
Product Specific Notices for Pax3 Antibody (HL160)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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