Pax5/BSAP Antibody (8F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005079-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pax5/BSAP Antibodies »

Product Specifications

Immunogen

PAX5 (NP_057953, 192 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADTNKRKRDEGIQESPVPNGHSLPGRDFLRKQMRGDLFTQQQLEVLDRVFERQHYSDIFTTTEPIKPEQTTEYSAMASLAGGLDDMKANLASPTPADIGSSVPGPQSYPI

Specificity

PAX5 - paired box gene 5 (B-cell lineage specific activator)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Pax5/BSAP Antibody (8F9)

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01]

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01]

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 expression in transfected 293T cell line by PAX5 monoclonal antibody (M01), clone 8F9.Lane 1: PAX5 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (8F9) [H00005079-M01]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (8F9) [H00005079-M01]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of monoclonal antibody to PAX5 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.
Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01]

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01]

Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 over-expressed 293 cell line, cotransfected with PAX5 Validated Chimera RNAi ( Cat # H00005079-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAX5 monoclonal antibody (M01), clone 8F9 (Cat # H00005079-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for Pax5/BSAP Antibody (8F9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pax5/BSAP

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Alternate Names

BSAP

Entrez Gene IDs

5079 (Human)

Gene Symbol

PAX5

OMIM

167414 (Human)

UniProt

NP_057953 (Human)

Additional Pax5/BSAP Products

Product Documents for Pax5/BSAP Antibody (8F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pax5/BSAP Antibody (8F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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