Skip to main content

Pax6 Antibody (PAX6/1166) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47915

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-47915-0.1mg

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAX6/1166

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367)

Localization

Nuclear

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44576).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Pax6 Antibody (PAX6/1166) - Azide and BSA Free

Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915]

Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915]

Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/1166) - Azide and BSA Free [NBP2-47915] - Human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (PAX6/1166).

Applications for Pax6 Antibody (PAX6/1166) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

Additional Pax6 Products

Product Documents for Pax6 Antibody (PAX6/1166) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pax6 Antibody (PAX6/1166) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...