Pax7 Antibody (1E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005081-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pax7 Antibodies »

Product Specifications

Immunogen

PAX7 (NP_002575.1, 411 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQSECLVPWASPVPIPSPTPRASCLFMESYKVVSGWGMSISQMEKLKSSQMEQFT

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PAX7 - paired box gene 7

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Pax7 Antibody (1E12)

Western Blot: Pax7 Antibody (1E12) [H00005081-M05]

Western Blot: Pax7 Antibody (1E12) [H00005081-M05]

Western Blot: Pax7 Antibody (1E12) [H00005081-M05] - Analysis of PAX7 expression in NIH/3T3 (Cat # L018V1).
Immunohistochemistry-Paraffin: Pax7 Antibody (1E12) [H00005081-M05]

Immunohistochemistry-Paraffin: Pax7 Antibody (1E12) [H00005081-M05]

Immunohistochemistry-Paraffin: Pax7 Antibody (1E12) [H00005081-M05] - Analysis of monoclonal antibody to PAX7 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 0.5 ug/ml
ELISA: Pax7 Antibody (1E12) [H00005081-M05]

ELISA: Pax7 Antibody (1E12) [H00005081-M05]

ELISA: Pax7 Antibody (1E12) [H00005081-M05] - Pax7 Antibody (1E12) [H00005081-M05] - Detection limit for recombinant GST tagged PAX7 is approximately 3ng/ml as a capture antibody.

Applications for Pax7 Antibody (1E12)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pax7

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Long Name

Paired Box Gene 7

Alternate Names

HUP1, RMS2

Entrez Gene IDs

5081 (Human)

Gene Symbol

PAX7

OMIM

167410 (Human)

UniProt

NP_002575 (Human)

Additional Pax7 Products

Product Documents for Pax7 Antibody (1E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pax7 Antibody (1E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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