PAX8 Antibody

Chromatin Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440]

Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440]

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Rat thyroid gland. PAX8 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440]

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - DLD1 xenograft . PAX8 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440]

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Mouse liver. PAX8 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: PAX8 Antibody [NBP1-32440]

Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE gel.

Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440] -

Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440] - Differentiation of human iPSC clone IV towards renal commitment.(a) Representative immunofluorescence images of co-staining for Lhx1/Osr1 up to day 6. (b) Images of co-staining of Pax2/Six2, Pax8/Six2 & Six2/Wt1 from day 6 to 12. (c) Expression of renal progenitor markers such as CD24, Claudin1 & GGT1 from day 0 to 19. Nuclei are stained with DAPI (blue). Scale bars: 50 μm (a, b), 20 μm (c). (d) Gene expression analysis for renal progenitor markers at different points in time. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] -

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - PDX model establishment from ccRCC injection. a Hematoxylin & Eosin staining of human tumor engrafted in murine models. A epresentative image of kidney PDX reported. Microscope Nikon Eclipse E1000 10X & 20X (b) Graph reporting % of patients who engrafted when orthotopically injected in mice & distributed following grading (3 G1; 7 G2; 13 G3; 7 G4). Eight mice for each patient injected & all 18 tumors on 30 which evaluated as engrafted developed tumor masses on ≥ of 6 mice. Tumors declared unable to engraft did not produce, at all, tumor masses. c Representative images (1 × 0.63) of PDXs excised 90 days after injection by Stereomicroscope (Olympus SZX10,XCX50). One representative image for G2, G3 & G4 types reported. d Representative images (1 × 0.63 & 1.25) of aberrant neo-angiogenesis formation in PDXs by Stereomicroscope (Olympus SZX10, XCX50 camera). e Hematoxylin & Eosin staining of PDXs versus parental primary patients. One representative tumor for each grade (G2, G3, G4) reported. Staining executed on OCT frozen samples. f Hematoxylin & Eosin & anti-PAX8, CD10, Vimentin & EMA staining reported on formalin-fixed & paraffin- embedded parental primary, metastatic tissues & PDXs. Primary, metastatic & PDX tissues obtained & shown from one representative patient. Microscope Nikon Eclipse 55i, magnification 20×. g Histogram showing number of engrafting tumor populations evaluated over 30 injected patient samples & correlated w/ patient recurrence frequency calculated as development of metastases after surgery. Orange color represents recurrent (n = 7) & metastatic (n = 3) patients in engrafted group (n = 18). Pink color represents recurrent patients (n = 2) in non-engrafted group (n = 12) for a total of 30 injected samples Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/30185225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PAX8 Antibody [NBP1-32440] -

Western Blot: PAX8 Antibody [NBP1-32440] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (NBP1-32440) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] -

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - PAX8 antibody detects PAX8 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.PAX8 stained by PAX8 antibody (NBP1-32440) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: PAX8 Antibody [NBP1-32440] -

Western Blot: PAX8 Antibody [NBP1-32440] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (NBP1-32440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.