Paxillin [p Tyr118] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24459

Novus Biologicals, part of Bio-Techne
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NBP2-24459
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Chicken, Primate, Rabbit, Rhesus Macaque, Xenopus, Zebrafish

Cited:

Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Paxillin Antibodies »

Product Specifications

Immunogen

A portion of human Paxillin protein containing a phosphorylated tyrosine residue at position 118 was used as the immunogen for this phospho antibody.

Reactivity Notes

Opossum, Chimpanzee, Orangutan, Salmon (100%), Tetradon (85%).

Modification

p Tyr118

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Paxillin [p Tyr118] Antibody

Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459]

Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459]

Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459] - Analysis of phospho Paxillin (Y118) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 118 and 2) an unphosphorylated tyrosine at position 118, using paxillin phospho antibody at 0.025 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for Paxillin [p Tyr118] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 30635353)

Western Blot

0.025-0.1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

UniProt

Q9DDK9 (Human)

Additional Paxillin Products

Product Documents for Paxillin [p Tyr118] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Paxillin [p Tyr118] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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