Paxillin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57097

Novus Biologicals, part of Bio-Techne
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NBP2-57097
NBP2-57097-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Paxillin Antibodies »

Product Summary for Paxillin Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ALNGTILDPLDQWQPSGSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSL

Reactivity Notes

Mouse 83%, Rat 84%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Paxillin Antibody

Western Blot: Paxillin Antibody [NBP2-57097]

Western Blot: Paxillin Antibody [NBP2-57097]

Western Blot: Paxillin Antibody [NBP2-57097] - Analysis in human cell line U-87 MG.
Immunocytochemistry/ Immunofluorescence: Paxillin Antibody [NBP2-57097]

Immunocytochemistry/ Immunofluorescence: Paxillin Antibody [NBP2-57097]

Immunocytochemistry/Immunofluorescence: Paxillin Antibody [NBP2-57097] - Staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites. Antibody staining is shown in green.

Applications for Paxillin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

1:250 - 1:500
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Paxillin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Paxillin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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