PBEF/Visfatin/NAMPT Antibody (BLR058F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76368

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76368
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR058F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PBEF/Visfatin Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 400 and 450

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PBEF/Visfatin/NAMPT Antibody (BLR058F)

Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368]

Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368]

Western Blot: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human and mouse NAMPT/PBEF/Visfatin by WB of RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 lysate. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH.
Immunocytochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368]

Immunocytochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368]

Immunocytochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE NCI-H460 by ICC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368]

Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368]

Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody (BLR058F) [NBP2-76368] - Detection of human NAMPT/PBEF/Visfatin in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal [BLR058F] (NBP2-76368). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for PBEF/Visfatin/NAMPT Antibody (BLR058F)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

5 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Phosphate Buffered Saline (PBS), BSA-Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: PBEF/Visfatin

PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Gene Symbol

NAMPT

Additional PBEF/Visfatin Products

Product Documents for PBEF/Visfatin/NAMPT Antibody (BLR058F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBEF/Visfatin/NAMPT Antibody (BLR058F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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