PCAF Antibody (5E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00008850-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCAF Antibodies »

Product Specifications

Immunogen

PCAF (AAH60823, 353 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPME

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PCAF (5E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PCAF Antibody (5E5)

Western Blot: PCAF Antibody (5E5) [H00008850-M06]

Western Blot: PCAF Antibody (5E5) [H00008850-M06]

Western Blot: PCAF Antibody (5E5) [H00008850-M06] - Analysis of PCAF expression in human thyroid (diffuse hyperplasia).
Immunocytochemistry/ Immunofluorescence: PCAF Antibody (5E5) [H00008850-M06]

Immunocytochemistry/ Immunofluorescence: PCAF Antibody (5E5) [H00008850-M06]

Immunocytochemistry/Immunofluorescence: PCAF Antibody (5E5) [H00008850-M06] - Analysis of monoclonal antibody to PCAF on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: PCAF Antibody (5E5) [H00008850-M06]

Western Blot: PCAF Antibody (5E5) [H00008850-M06]

Western Blot: PCAF Antibody (5E5) [H00008850-M06] - Analysis of PCAF expression in C32 (Cat # L002V1).
View 5 more images

Applications for PCAF Antibody (5E5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCAF

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Long Name

p300/CBP-associated Factor

Alternate Names

GCN5, KAT2B

Entrez Gene IDs

8850 (Human)

Gene Symbol

KAT2B

OMIM

602303 (Human)

UniProt

AAH60823 (Human)

Additional PCAF Products

Product Documents for PCAF Antibody (5E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCAF Antibody (5E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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