PCBP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19715

Novus Biologicals, part of Bio-Techne
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NBP2-19715
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCBP2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCBP2. The exact sequence is proprietary.

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCBP2 Antibody

Western Blot: PCBP2 Antibody [NBP2-19715]

Western Blot: PCBP2 Antibody [NBP2-19715]

Western Blot: PCBP2 Antibody [NBP2-19715] - CBP2 antibody detects PCBP2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with PCBP2 antibody diluted by 1:1000
Immunocytochemistry/ Immunofluorescence: PCBP2 Antibody [NBP2-19715]

Immunocytochemistry/ Immunofluorescence: PCBP2 Antibody [NBP2-19715]

Immunocytochemistry/Immunofluorescence: PCBP2 Antibody [NBP2-19715] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCBP2 protein stained by PCBP2 antibody diluted at 1:750. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: PCBP2 Antibody [NBP2-19715]

Immunohistochemistry-Paraffin: PCBP2 Antibody [NBP2-19715]

Immunohistochemistry-Paraffin: PCBP2 Antibody [NBP2-19715] - Mouse brain. PCBP2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for PCBP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCBP2

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, heterogenous nuclear ribonucleoprotein E2, hnRNP E2, hnRNP-E2, HNRPE2, MGC110998, poly(rC) binding protein 2, poly(rC)-binding protein 2

Gene Symbol

PCBP2

UniProt

Q15366 (Human)

Additional PCBP2 Products

Product Documents for PCBP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCBP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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