PCCA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005095-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bacteria

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCCA Antibodies »

Product Specifications

Immunogen

PCCA (AAH00140.1, 1 a.a. - 703 a.a.) full-length human protein. MLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Specificity

PCCA - propionyl Coenzyme A carboxylase, alpha polypeptide,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for PCCA Antibody

Western Blot: PCCA Antibody [H00005095-B01P]

Western Blot: PCCA Antibody [H00005095-B01P]

Western Blot: PCCA Antibody [H00005095-B01P] - Analysis of PCCA expression in human liver.
Western Blot: PCCA Antibody [H00005095-B01P]

Western Blot: PCCA Antibody [H00005095-B01P]

Western Blot: PCCA Antibody [H00005095-B01P] - Analysis of PCCA expression in U-2 OS.
Western Blot: PCCA Antibody [H00005095-B01P]

Western Blot: PCCA Antibody [H00005095-B01P]

Western Blot: PCCA Antibody [H00005095-B01P] - Analysis of PCCA expression in transfected 293T cell line by PCCA polyclonal antibody. Lane 1: PCCA transfected lysate(77.33 KDa). Lane 2: Non-transfected lysate.

Applications for PCCA Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCCA

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

EC 6.4.1, EC 6.4.1.3, pccA complementation group, PCCase alpha subunit, PCCase subunit alpha, propanoyl-CoA:carbon dioxide ligase alpha subunit, Propanoyl-CoA:carbon dioxide ligase subunit alpha, propionyl CoA carboxylase, alpha polypeptide, propionyl Coenzyme A carboxylase, alpha polypeptide, propionyl-CoA carboxylase alpha chain, mitochondrial

Entrez Gene IDs

5095 (Human)

Gene Symbol

PCCA

UniProt

AAH00140.1 (Human)

Additional PCCA Products

Product Documents for PCCA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCCA Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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