PCDHGA5 Antibody (5H5)

Novus Biologicals, part of Bio-Techne | Catalog # H00056110-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCDHGA5 Antibodies »

Product Specifications

Immunogen

PCDHGA5 (NP_061741, 205 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETVHDLLLTALDGGDPVLSGTTHIRVTVLDANDNAPLFTPSEYSVSVPENIPVGTRLLMLTATDPDEGINGKLTYSFRNEEEKISETFQLDSNLGEISTL

Reactivity Notes

Human. Other species not tested.

Specificity

PCDHGA5 - protocadherin gamma subfamily A, 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PCDHGA5 Antibody (5H5)

Western Blot: PCDHGA5 Antibody (5H5) [H00056110-M01]

Western Blot: PCDHGA5 Antibody (5H5) [H00056110-M01]

Western Blot: PCDHGA5 Antibody (5H5) [H00056110-M01] - Analysis of PCDHGA5 expression in transfected 293T cell line by PCDHGA5 monoclonal antibody (M01), clone 5H5.Lane 1: PCDHGA5 transfected lysate(100.935 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PCDHGA5 Antibody (5H5) [H00056110-M01] - Detection limit for recombinant GST tagged PCDHGA5 is approximately 0.1ng/ml as a capture antibody.

Applications for PCDHGA5 Antibody (5H5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCDHGA5

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Alternate Names

cadherin ME3, CDH-GAMMA-A5, ME3, MGC142020, PCDH-GAMMA-A5, protocadherin gamma subfamily A, 5, protocadherin gamma-A5

Entrez Gene IDs

56110 (Human)

Gene Symbol

PCDHGA5

OMIM

606292 (Human)

UniProt

NP_061741 (Human)

Additional PCDHGA5 Products

Product Documents for PCDHGA5 Antibody (5H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCDHGA5 Antibody (5H5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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