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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1505 and 1555 of human Cleavage and Polyadenylation Factor II Subunit using the numbering given in entry NP_056969.2 (GeneID 51585).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCF11 Antibody

Western Blot: PCF11 Antibody [NBP2-12787]

Western Blot: PCF11 Antibody [NBP2-12787]

Western Blot: PCF11 Antibody [NBP2-12787] - Detection of human PCF11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PCF11 antibody NBP2-12787 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PCF11 was also immunoprecipitated by rabbit anti-PCF11 antibody NBP2-12786, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Applications for PCF11 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCF11

PCF11 is a component of pre-mRNA cleavage complex II

Long Name

Pre-mRNA cleavage complex 2 protein Pcf11

Alternate Names

KIAA0824

Gene Symbol

PCF11

Additional PCF11 Products

Product Documents for PCF11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCF11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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