PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24070
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-PCGF3-1D5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human PCGF3 protein
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free
Immunohistochemistry-Paraffin: PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free [NBP3-24070] -
Formalin-fixed, paraffin-embedded human prostate stained with PCGF3 antibody (PCRP-PCGF3-1D5) - Azide and BSA Free HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Protein Array: PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free [NBP3-24070] -
Analysis of Protein Array containing more than 19,000 full-length human proteins using PCGF3 antibody (PCRP-PCGF3-1D5) - Azide and BSA Free
Flow Cytometry: PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free [NBP3-24070] -
Flow Cytometric Analysis of PFA-fixed HeLa cells. PCGF3 antibody (PCRP-PCGF3-1D5) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).Applications for PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PCGF3
Alternate Names
DKFZp686D20235, DONG1, FLJ36550, FLJ43813, MGC129615, MGC40413, polycomb group ring finger 3, polycomb group RING finger protein 3, ring finger protein 3, RNF3, RNF3ARING finger protein 3A
Gene Symbol
PCGF3
Additional PCGF3 Products
Product Documents for PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free
Product Specific Notices for PCGF3 Antibody (PCRP-PCGF3-1D5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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