PCK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80927

Novus Biologicals, part of Bio-Techne
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NBP1-80927
NBP1-80927-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCK1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCK1 Antibody

Western Blot: PCK1 Antibody [NBP1-80927]

Western Blot: PCK1 Antibody [NBP1-80927]

Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in human kidney tissue.
Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80927]

Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80927]

Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80927] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.
Western Blot: PCK1 Antibody [NBP1-80927]

Western Blot: PCK1 Antibody [NBP1-80927]

Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in control (vector only transfected HEK293T lysate) and PCK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for PCK1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCK1

The PCK1 gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expressio

Long Name

Phosphoenolpyruvate Carboxykinase 1

Alternate Names

PEPCK-C, PEPCK1

Entrez Gene IDs

5105 (Human)

Gene Symbol

PCK1

UniProt

P35558 (Human)

Additional PCK1 Products

Product Documents for PCK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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