PCNA Antibody (rPCNA/8858)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23669
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rPCNA/8858
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human PCNA protein
Localization
Nucleus. Cytoplasm.
Marker
G1- & S-phase Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for PCNA Antibody (rPCNA/8858)
Immunohistochemistry-Paraffin: PCNA Antibody (rPCNA/8858) [NBP3-23669] -
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (rPCNA/8858) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: PCNA Antibody (rPCNA/8858) [NBP3-23669] -
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (rPCNA/8858) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for PCNA Antibody (rPCNA/8858)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for PCNA Antibody (rPCNA/8858)
Product Specific Notices for PCNA Antibody (rPCNA/8858)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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