PCNA Antibody (SPM350)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32989
![Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989]](https://resources.bio-techne.com/images/products/PCNA-Antibody-SPM350-Immunohistochemistry-Paraffin-NBP2-32989-img0002.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Chicken, Drosophila, Monkey, S. pombe, Yeast, Zebrafish
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM350
Concentration
0.2 mg/ml
Product Summary for PCNA Antibody (SPM350)
Immunogen
Rat PCNA/protein A fusion protein (Uniprot: P12004)
Reactivity Notes
Yeast (S. pombe. S. cerevisiae).
Localization
Predominantly nuclear, some cytoplasmic
Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This monoclonal antibody is excellent for multiple applications.
Marker
G1- & S-phase Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34767)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for PCNA Antibody (SPM350)
Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989]
Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).Applications for PCNA Antibody (SPM350)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
UniProt
Additional PCNA Products
Product Documents for PCNA Antibody (SPM350)
Product Specific Notices for PCNA Antibody (SPM350)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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