PCNA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-456

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat

Predicted:

Bovine (100%), Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PCNA Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 75-125 of human proliferating cell nuclear antigen using the SwissProt entry P12004 (GeneID 5111).

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA Antibody

Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456]

Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PCNA Antibody [NB100-456]

Immunohistochemistry: PCNA Antibody [NB100-456]

Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB
Flow Cytometry: PCNA Antibody [NB100-456]

Flow Cytometry: PCNA Antibody [NB100-456]

Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.
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Applications for PCNA Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCNA

PCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor for DNA polymerase delta. It has also been shown to interact with several other proteins that are involved in DNA replication and repair, including replication factor C, DNA ligase I, XPG, FEN1 and p21. This particular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Entrez Gene IDs

5111 (Human)

Gene Symbol

PCNA

UniProt

P12004 (Human)

Additional PCNA Products

Product Documents for PCNA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCNA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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