PCNA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32075

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-32075
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila, Golden Syrian Hamster

Cited:

Mouse

Predicted:

Bovine (98%), Porcine (99%), Rhesus Macaque (100%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In-situ Hybridization, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCNA Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (88%).

Localization

Nucleus

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCNA Antibody

Western Blot: PCNA Antibody [NBP1-32075]

Western Blot: PCNA Antibody [NBP1-32075]

Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500
Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-32075]

Immunocytochemistry/ Immunofluorescence: PCNA Antibody [NBP1-32075]

Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um
Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075]

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse liver. PCNA antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 9 more images

Applications for PCNA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCNA

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCNA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.