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PCNA associated factor Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80555

Novus Biologicals, part of Bio-Techne
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NBP1-80555

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the N terminal of human KIAA0101. Peptide sequence MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKEHVLCNLI. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PCNA associated factor Antibody

Western Blot: PCNA associated factor Antibody [NBP1-80555]

Western Blot: PCNA associated factor Antibody [NBP1-80555]

Western Blot: PCNA associated factor Antibody [NBP1-80555] - KIAA0101 tv2 is highly expressed in adjacent non-tumorous liver tissues (NTs) compared to hepatocellular carcinoma (HCC) tissues(A-B) KIAA0101 tv2 protein levels were analyzed by western blot in 42 pairs of HCC cases and NTs. The graph shows the mean+-standard error of mean (SEM) of KIAA0101 tv2 protein overexpression rates by western blot. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/16702) licensed under a CC-BY license.
Western Blot: PCNA associated factor Antibody [NBP1-80555]

Western Blot: PCNA associated factor Antibody [NBP1-80555]

Western Blot: PCNA associated factor Antibody [NBP1-80555] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
PCNA associated factor Antibody

Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP1-80555] -

Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP1-80555] - KIAA0101 tv2 is highly expressed in adjacent non-tumorous liver tissues (NTs) compared to hepatocellular carcinoma (HCC) tissues(A-B) KIAA0101 tv2 protein levels were analyzed by western blot in 42 pairs of HCC cases & NTs. The graph shows the mean±standard error of mean (SEM) of KIAA0101 tv2 protein overexpression rates by western blot. (C-D) All tissues were assessed by immunohistochemistry (IHC) using anti-KIAA0101 tv2 polyclonal antibody. Representative images are shown (Original magnification ×400). Each bar represents results from three independent experiments. ***P<0.005. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28410205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PCNA associated factor Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Application Notes
Use in Immunohistochemistry paraffin reported in scientific literature (PMID 28410205).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA associated factor

KIAA0101/NS5ATP9/PAF/p15(PAF) is involved in protection of cells from UV-induced cell death and is also a Hepatitis C NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma.

Alternate Names

HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, KIAA0101, NS5ATP9p15PAF, OEATC-1OEATC1, Overexpressed in anaplastic thyroid carcinoma 1, p15(PAF), PAF, PCNA-associated factor

Gene Symbol

PCLAF

Additional PCNA associated factor Products

Product Documents for PCNA associated factor Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCNA associated factor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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