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PCNP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86312

Novus Biologicals, part of Bio-Techne
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NBP1-86312

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NEDEDSEPEEMPPEAKMRMKNIGRDTPTSAGPNSFNKGKHGFSDNQKLWERNIKSHLGNVHDQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNP Antibody

Western Blot: PCNP Antibody [NBP1-86312]

Western Blot: PCNP Antibody [NBP1-86312]

Western Blot: PCNP Antibody [NBP1-86312] - Analysis in control (vector only transfected HEK293T lysate) and PCNP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PCNP Antibody [NBP1-86312]

Immunocytochemistry/ Immunofluorescence: PCNP Antibody [NBP1-86312]

Immunocytochemistry/Immunofluorescence: PCNP Antibody [NBP1-86312] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PCNP Antibody [NBP1-86312]

Immunohistochemistry-Paraffin: PCNP Antibody [NBP1-86312]

Immunohistochemistry-Paraffin: PCNP Antibody [NBP1-86312] - Staining of human colon shows strong nuclear positivity in glandular cells.

Applications for PCNP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNP

PCNP may be involved in cell cycle regulation

Alternate Names

DKFZp781I24156, PEST proteolytic signal containing nuclear protein, PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

Gene Symbol

PCNP

Additional PCNP Products

Product Documents for PCNP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCNP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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