PCNP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37692
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody maps to a region between residue 1 to 50 of human PEST Proteolytic Signal Containing Nuclear Protein using the numbering given in entry NP_065090.1 (GeneID 57092).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PCNP Antibody
Western Blot: PCNP Antibody [NBP2-37692]
Western Blot: PCNP Antibody [NBP2-37692] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: PCNP Antibody [NBP2-37692]
Immunoprecipitation: PCNP Antibody [NBP2-37692] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for IP at 6 ug per reaction. PCNP was also immunoprecipitated by rabbit anti-PCNP antibody. For blotting immunoprecipitated PCNP, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for PCNP Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:10000 - 1:25000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PCNP
Alternate Names
DKFZp781I24156, PEST proteolytic signal containing nuclear protein, PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
Gene Symbol
PCNP
UniProt
Additional PCNP Products
Product Documents for PCNP Antibody
Product Specific Notices for PCNP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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