PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65332
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # TY25
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
B7-DC transfected RAW264.7 cells.
Specificity
NB100-65332 recognizes mouse CD273, a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Antibodies produced by clone TY25 are reported to block the binding of CD273 (B7-DC) to it's receptor.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free
![Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free [NB100-65332]](https://resources.bio-techne.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-TY25-Flow-Cytometry-NB100-65332-img0003.jpg "Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free [NB100-65332]")
Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free [NB100-65332]
Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) [NB100-65332] - Analysis using the Biotin conjugate of NB100-65332. Staining of B7-DC transfected cells with Anti-Mouse CD273 (B7-DC) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.![Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free [NB100-65332]](https://resources.bio-techne.com/images/products/PD-L2-B7-DC-PDCD1LG2-Antibody-TY25-Flow-Cytometry-NB100-65332-img0004.jpg "Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free [NB100-65332]")
Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free [NB100-65332]
Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) [NB100-65332] - Analysis using the PE conjugate of NB100-65332. Staining of non-transfected (left) and mouse B7-DC-transfected (right) cells with 0.25 ug of Rat IgG2a kappa Isotype Control PE (NBP1-43780) (open histogram) or 0.06 ug of Anti-Mouse CD273 (B7-DC) PE (filled histogram).Applications for PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:100-1:200
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PD-L2/B7-DC
Long Name
Programmed Death-1 Ligand-2
Alternate Names
B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2
Gene Symbol
PDCD1LG2
Additional PD-L2/B7-DC Products
Product Documents for PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free
Product Specific Notices for PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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