PDCD4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76738

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PDCD4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human PDCD4. The immunogen is located within the last 50 amino acids of PDCD4. Amino Acid Squence: GRKRFVSEGDGGRLKPESY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDCD4 Antibody - BSA Free

Western Blot: PDCD4 AntibodyBSA Free [NBP1-76738]

Western Blot: PDCD4 AntibodyBSA Free [NBP1-76738]

Western Blot: PDCD4 Antibody [NBP1-76738] - A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1ug/ml.
Immunohistochemistry-Paraffin: PDCD4 Antibody - BSA Free [NBP1-76738]

Immunohistochemistry-Paraffin: PDCD4 Antibody - BSA Free [NBP1-76738]

Immunohistochemistry-Paraffin: PDCD4 Antibody [NBP1-76738] - Mouse brain tissue with PDCD4 antibody at 2.5 ug/ml.
PDCD4 Antibody - BSA Free

Immunohistochemistry: PDCD4 Antibody - BSA Free [NBP1-76738] -

Immunohistochemistry: PDCD4 Antibody - BSA Free [NBP1-76738] - Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 u/mL.
View 1 more images

Applications for PDCD4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDCD4

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

Long Name

Programmed Cell Death 4

Alternate Names

H731, Protein 197/15a

Entrez Gene IDs

27250 (Human); 18569 (Mouse); 64031 (Rat)

Gene Symbol

PDCD4

UniProt

B5ME91 (Human)

Additional PDCD4 Products

Product Documents for PDCD4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDCD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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