PDE1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51996

Novus Biologicals, part of Bio-Techne
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NBP1-51996
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PDE1A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLHKNSEDLVNAE corresponding to C-Terminus according to NP_005010.2.

Epitope

C-DLHKNSEDLVNAE

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Specificity

This antibody is expected to recognize isoform 1 (NP_005010.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PDE1A Antibody

Western Blot: PDE1A Antibody [NBP1-51996]

Western Blot: PDE1A Antibody [NBP1-51996]

Western Blot: PDE1A Antibody [NBP1-51996] - 3.75ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: PDE1A Antibody [NBP1-51996]

Western Blot: PDE1A Antibody [NBP1-51996]

Western Blot: PDE1A Antibody [NBP1-51996] - Western blot analysis of PDE1A in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-51996 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PDE1A Antibody

Application
Recommended Usage

Immunohistochemistry

1-3ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1:100-1:2000
Application Notes
Western blot: Approx 55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 62.3kDa according to NP_005010.2). Primary incubation was 1 hour
IHC: Paraffin embedded Human Kidney.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE1A

PDE1A, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, B, and C). PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. The PDE1A gene family includes various proteins that differ from each other at amino and carboxy termini of the protein.

Long Name

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

Alternate Names

61 kDa Cam-PDE, Cam-PDE 1A, hCam-1

Entrez Gene IDs

5136 (Human); 18573 (Mouse); 81529 (Rat)

Gene Symbol

PDE1A

UniProt

NP_005010.2 (Human)

Additional PDE1A Products

Product Documents for PDE1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDE1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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