PDE1B Antibody (10B10B12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37403

Novus Biologicals, part of Bio-Techne
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NBP2-37403
NBP2-37403-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey, Primate

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10B10B12

Format

BSA Free

Concentration

1 mg/ml

View all PDE1B Antibodies »

Product Summary for PDE1B Antibody (10B10B12) - BSA Free

Immunogen

Purified recombinant fragment of human PDE1B (AA: 370-536) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE1B Antibody (10B10B12) - BSA Free

Western Blot: PDE1B Antibody (10B10B12)BSA Free [NBP2-37403]

Western Blot: PDE1B Antibody (10B10B12)BSA Free [NBP2-37403]

Western Blot: PDE1B Antibody (10B10B12) [NBP2-37403] - Western blot analysis using PDE1B mouse mAb against A549 (1), SK-MES-1 (2), PC-12 (4),3T3L1 (5) cell lysate and Mouse brain (3) tissue lysate.
Flow Cytometry: PDE1B Antibody (10B10B12) - BSA Free [NBP2-37403]

Flow Cytometry: PDE1B Antibody (10B10B12) - BSA Free [NBP2-37403]

Flow Cytometry: PDE1B Antibody (10B10B12) [NBP2-37403] - Flow cytometric analysis of A549 cells using PDE1B mouse mAb (green) and negative control (red).
Western Blot: PDE1B Antibody (10B10B12)BSA Free [NBP2-37403]

Western Blot: PDE1B Antibody (10B10B12)BSA Free [NBP2-37403]

Western Blot: PDE1B Antibody (10B10B12) [NBP2-37403] - Western blot analysis using PDE1B mAb against human PDE1B recombinant protein. (Expected MW is 44.4 kDa)
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Applications for PDE1B Antibody (10B10B12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE1B

PDE1B, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The tissue distribution of PDE1B is different from other PDE1 gene family members. In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids.

Long Name

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Alternate Names

63 kDa Cam-PDE, Cam-PDE 1B, PDES1B

Gene Symbol

PDE1B

Additional PDE1B Products

Product Documents for PDE1B Antibody (10B10B12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDE1B Antibody (10B10B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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