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PDE3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46182

Novus Biologicals, part of Bio-Techne
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NBP1-46182

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 575 and 625 of human Phosphodiesterase 3A using the numbering given in entry NP_000912.3 (GeneID 5139).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE3A Antibody

Western Blot: PDE3A Antibody [NBP1-46182]

Western Blot: PDE3A Antibody [NBP1-46182]

Western Blot: PDE3A Antibody [NBP1-46182] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3A antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PDE3A was also immunoprecipitated by rabbit anti-PDE3A antibody NBP1-46181, which recognizes an upstream epitope.

Applications for PDE3A Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDE3A

Phosphodiesterase 3A is a cGMP inhibited, cAMP/cGMP dual-specific phosphodiesterase. PDE3A has been implicated in male erectile dysfunction, mammalian oocyte maturation, platelet function, vascular smooth muscle function, and salivary gland tumors. Three alternatively spliced transcripts have been reported.

Long Name

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Alternate Names

cGI-PDE, CGI-PDE A

Gene Symbol

PDE3A

Additional PDE3A Products

Product Documents for PDE3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDE3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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