PDE3A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19744
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PDE3A. The exact sequence is proprietary.
Reactivity Notes
Pig (88%). Rat reactivity reported from a verified customer review.
Localization
Membrane; Peripheral membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PDE3A Antibody
![Western Blot: PDE3A Antibody [NBP2-19744]](https://resources.bio-techne.com/images/products/PDE3A-Antibody-Western-Blot-NBP2-19744-img0002.jpg "Western Blot: PDE3A Antibody [NBP2-19744]")
Western Blot: PDE3A Antibody [NBP2-19744]
Western Blot: PDE3A Antibody [NBP2-19744] - Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5 nM and 25 nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6). Loading of all 293T cell lysates are ~30 ug. H9C2 lysate is ~7 ug due to limited materials. Antibody at 1:500. Secondary antibody at 1:10,000. Blocked with 5% BSA. WB image submitted by a verified customer review.![Western Blot: PDE3A Antibody [NBP2-19744]](https://resources.bio-techne.com/images/products/PDE3A-Antibody-Western-Blot-NBP2-19744-img0001.jpg "Western Blot: PDE3A Antibody [NBP2-19744]")
Western Blot: PDE3A Antibody [NBP2-19744]
Western Blot: PDE3A Antibody [NBP2-19744] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.Applications for PDE3A Antibody
Application
Recommended Usage
Western Blot
1:500 - 1:3000
Application Notes
PDE3A antibody validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 4 using NBP2-19744 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PDE3A
Long Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Alternate Names
cGI-PDE, CGI-PDE A
Gene Symbol
PDE3A
UniProt
Additional PDE3A Products
Product Documents for PDE3A Antibody
Product Specific Notices for PDE3A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.