PDE3B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46183
![Western Blot: PDE3B Antibody [NBP1-46183]](https://resources.bio-techne.com/images/products/PDE3B-Antibody-Western-Blot-NBP1-46183-img0005.jpg "Western Blot: PDE3B Antibody [NBP1-46183]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for PDE3B Antibody
Immunogen
The immunogen for this product maps to a region between residue 500 and 550 of human Phosphodiesterase 3B using the numbering given in entry NP_000913.2 (GeneID 5140).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PDE3B Antibody
Western Blot: PDE3B Antibody [NBP1-46183]
Western Blot: PDE3B Antibody [NBP1-46183] - Detection of Human PDE3B by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3B antibody NBP1-46183 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PDE3B was also immunoprecipitated by rabbit anti-PDE3B antibody NBP1-46184, which recognizes a downstream epitope. For blotting immunoprecipitated PDE3B, the blot Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for PDE3B Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDE3B
Long Name
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Alternate Names
CGI-PDE B, CGIP1, CGIPDE1
Gene Symbol
PDE3B
Additional PDE3B Products
Product Documents for PDE3B Antibody
Product Specific Notices for PDE3B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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