PDE4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2562

Novus Biologicals, part of Bio-Techne
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NB100-2562
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PDE4B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DIDIATEDKSPVDT corresponding to C-Terminus according to NP_002591.2, NP_001032418.1, NP_001032416.1, NP_001032417.1.

Specificity

This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE4B Antibody

Western Blot: PDE4B Antibody [NB100-2562]

Western Blot: PDE4B Antibody [NB100-2562]

Western Blot: PDE4B Antibody [NB100-2562] - Staining of Mouse Brain Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PDE4B Antibody [NB100-2562]

Immunohistochemistry-Paraffin: PDE4B Antibody [NB100-2562]

Immunohistochemistry-Paraffin: PDE4B Antibody [NB100-2562] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: PDE4B Antibody [NB100-2562]

Western Blot: PDE4B Antibody [NB100-2562]

Western Blot: PDE4B Antibody [NB100-2562] - HEK293 overexpressing Human PDE4B and probed with (mock transfection in first lane).

Applications for PDE4B Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 10 ug/ml
Application Notes
WB: Approx. 85 kDa band observed in mouse brain lysates (calculated MW of 83.3 kDa band according to human NP_002591.2 and 82.1 kDa band according to mouse NP_062814.2). In transfected HEK293 transiently expressing human PDE4B a band of approx. 85 kDa is observed. This band is not observed in the non-transfected HEK293.
IHC: Paraffin embedded Human Prostate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE4B

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Alternate Names

DPDE4, PDE32

Entrez Gene IDs

5142 (Human); 18578 (Mouse); 24626 (Rat)

Gene Symbol

PDE4B

UniProt

Q07343 (Human)

Additional PDE4B Products

Product Documents for PDE4B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDE4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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