PDE6D Antibody (5C7)

Novus Biologicals, part of Bio-Techne | Catalog # H00005147-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5C7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDE6D Antibodies »

Product Specifications

Immunogen

PDE6D (AAH07831, 1 a.a. ~ 150 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

Localization

Cytoplasmic

Specificity

PDE6D - phosphodiesterase 6D, cGMP-specific, rod, delta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDE6D Antibody (5C7)

Western Blot: PDE6D Antibody (5C7) [H00005147-M06]

Western Blot: PDE6D Antibody (5C7) [H00005147-M06]

Western Blot: PDE6D Antibody (5C7) [H00005147-M06] - Analysis of PDE6D expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: PDE6D Antibody (5C7) [H00005147-M06]

Immunocytochemistry/ Immunofluorescence: PDE6D Antibody (5C7) [H00005147-M06]

Immunocytochemistry/Immunofluorescence: PDE6D Antibody (5C7) [H00005147-M06] - Analysis of monoclonal antibody to PDE6D on HeLa cell. Antibody concentration 20 ug/ml
ELISA: PDE6D Antibody (5C7) [H00005147-M06]

ELISA: PDE6D Antibody (5C7) [H00005147-M06]

ELISA: PDE6D Antibody (5C7) [H00005147-M06] - Detection limit for recombinant GST tagged PDE6D is approximately 0.03ng/ml as a capture antibody.

Applications for PDE6D Antibody (5C7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDE6D

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alpha/beta) that is regulated by two inhibitory y subunits. As PDE6D does not modify the catalytic properties of PDEalpha/beta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

Alternate Names

GMP-PDE delta, PDED, Protein p17

Entrez Gene IDs

5147 (Human)

Gene Symbol

PDE6D

OMIM

602676 (Human)

UniProt

AAH07831 (Human)

Additional PDE6D Products

Product Documents for PDE6D Antibody (5C7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDE6D Antibody (5C7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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