PDE6D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32730

Novus Biologicals, part of Bio-Techne
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NBP1-32730
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Xenopus

Predicted:

Bovine (99%), Canine (98%), Rhesus Macaque (99%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PDE6D Antibodies »

Product Specifications

Immunogen

Full length human PDE6D Recombinant protein.

Reactivity Notes

Rat (85%). Xenopus reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE6D Antibody

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730]

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730]

Immunohistochemistry-Paraffin: PDE6D Antibody [NBP1-32730] - SAS xenograft, using PDE6D antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PDE6D Antibody [NBP1-32730]

Western Blot: PDE6D Antibody [NBP1-32730]

Western Blot: PDE6D Antibody [NBP1-32730] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with PDE6D antibody [N1C3] diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
PDE6D Antibody

Immunocytochemistry/Immunofluorescence: PDE6D Antibody [NBP1-32730] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDE6D stained by PDE6D antibody [N1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10μm

Immunocytochemistry/Immunofluorescence: PDE6D Antibody [NBP1-32730] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDE6D stained by PDE6D antibody [N1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um
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Applications for PDE6D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Reviewed Applications

Read 1 review rated 4 using NBP1-32730 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDE6D

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alpha beta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDE alpha beta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

Alternate Names

GMP-PDE delta, PDED, Protein p17

Gene Symbol

PDE6D

Additional PDE6D Products

Product Documents for PDE6D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDE6D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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